The Cells We Use
SCT’s process begins by taking a sample enriched in antibody secreting B cells, called plasma cells. Plasma cells have undergone affinity maturation, so their antibodies have been selected by the immune system to have higher affinity against their target. Single cells are deposited into thousands of wells in an addressable array.
Our Screening Method
The secreted antibodies from thousands of plasma cells are captured onto slides and the captured antibodies are interrogated for their binding activity. Antibodies can be screened to find relative affinity, subclass, cross-reactivity (e.g. Human and Cynomolgus), and more characteristics depending on your
Output from the use of AbTheneum™ is displayed as a compendium of Big Data in what is called the Antibody Atlas™. Each study focused on a different target protein has its own proprietary Antibody Atlas™.
The Antibody Atlas™ is a cross-reference of results that can include affinity, kinetics, epitope binding, functional assessment, DNA sequences of the antibody cognate light and heavy chains, and structural inference for each antibody. The resulting relational database presents an easy format in which to expand an antibody screening campaign, and pick the best candidates for downstream characterization.